Aim: Dental phobia stems from fear and anxiety and is considered a major hurdle in dental treatment of pediatric patients who in turn resist or start avoiding treatment. Endogenous indolamines like melatonin which is produced by pineal gland has a chief role in regulation of circadian rhythms. Melatonin acts like an oncostatic agent, known to possess antioxidant, anti-inflammatory as well as anticonvulsant property. It is mainly administered through oral or sublingual route of drug administration. No substantial side effects have been reported by administration of melatonin. Soon after melatonin intake, it undergoes first-pass effect reaching in about 60 min to its peak plasma levels and within 4 h the blood level falls. Study Design: The present case-control study is a retrospective study with age and sex matched recruitments. Data collection was done as per first visit and then post treatment of 50 subjects who reported to the Department of Preventive and Pediatric dentistry. Methods: In accord with the literature, 25 pediatric subjects were administered with 0.5 mg/ kg body weight Melatonin 60’ prior to their first visit followed by their Pedodontic management. As per record no initial Pedodontic treatment was given to the rest of the 25 recruits. The successful management of treatment was assessed by analyzing their pain experience through FLACC behavioral pain scale. Results: A statistically significant number of children (n=50) both age and sex matched were included in the study and further divided into Group A and Group B with mean age of 7.56 years and 7.08 years respectively. Group A received Melatonin treatment while Group B was not given any preventive treatment. As per the FLACC scale categories, Melatonin was well tolerated by all the children of A Group, out of which 42% of subjects showed relaxed levels or slight discomfort (0-3), 42% presented with moderate discomfort or pain (4-6) while only 16% showed severe or intense pain or discomfort (7-10). Conclusions: The present study thus establishes a positive role of Melatonin on Pedodontic therapy as it has a potent analgesic and anxiolytic potential. Limitation of study is smaller sample size.