This study was carried out using 36 paraffin block specimen collected from bone biopsies diagnosed in National Orthopaedic Hospital Enugu. Twenty four (24) of this blocks representing 66.6 % were from male patients while the other twelve (12) representing 33.3 % were from female patients. All the cases were diagnosed as osteosarcoma between January 2010 and June 2016. Five (5) out of the 36 blocks were used as controls. Duplicate (2) sections were made from each block and stained with H &E as well as Highman’scongo red technique. In demonstrating amyloid deposit, in the 36 test samples, 13.8% were positive for amyloid with H &E while 11.1% of the total 36 test samples were positive for amyloid with Highman’s Congo red technique. This test result shows a low incidence of amyloidosis in osteosarcoma. In addition, Congo red showed greater specificity, eliminating false positive result, hence a better stain in all amyloid study.