Objectives: Assess the diagnostic value of Bone Metabolism Biomarker Osteocalcin for Monitoring Treatment Chronic Periodontitis. Materials and Methods: 84 patients with mild chronic periodontitis were participated in this study who received initial periodontal therapy. All patients underwent a thorough clinical and laborator examination including a determination of the serum osteocalcin content, at the beginning of the study and 1, 3 months after therapy. Results: Healing period occurred without complications, and with minimal postoperative discomfort. The mean BOP before treatment was 2.5 ± 0.31, after 1 month treatment the mean BOP 1.7 ± 0.1, after 3 months treatment the mean BOP 1.3 ± 0.12(p>0.05). The probing depth according to the mean value (PD) was 5.27 ± 0.77 mm at the beginning of the study and, respectively, 4.35 ± 0.73 mm 1 month after therapy and 2.82 ± 0.43 (p> 0.05 ) 3 months after therapy.The clinical attachment level (CAL) mean value was 5.27 0.77 mm at baseline and,1 month after therapy it was was respectively, 4.35 0.73 mm and 3 month after therapy it was 2.82 0.43 (p 0.05 ).The osteocalcin in serum was 5.27 0.77 mm at baseline 10.58 ng/ml, 1 month after periodontal therapy it was 14.23, 3 month after and, 3 month after periodontal therapy it was was 17.74 ng/ml, As a result, there is a statistically significant increase in mean osteocalcin postoperatively (p 0.05). The study results showed that elevated serum osteocalcin concentration in patients with chronic periodontitis after periodontal therapy correlates with PPD and CAL, suggesting that its role could be used as a diagnostic test for patient and to complement other diagnostic tests. Conclusions: The results of this study showed that a combination treatment with the use of bone graft materials, hyaluronic acid and magnetic laser therapy is an effective therapy for the treatment of periodontal disease.