According to a Mutawatir Hadith of the Noble Prophet ( صلى الله عليه وسلم ) it was established Glorious Qur'an revealed with seven letters. There are various opinions of scholars about the meaning of these letters, this paper discussing the opinion and categorized them based on their strength of evidence into three namely; opinion which have no basis whatsoever and opinions which have some apparent basis but a weak opinions, and opinion that has relevance going with majority scholars of Qur'anic science. It is highlighted the relationship between the seven letters and seven Qira'at and them clarify those misunderstood it and finally discuss its wisdom in relation to proper recitation of Glorious Qur'an.