The study was done to determine the prevalence of Variant Creutzfeldt-Jakob disease prion among blood donors in Owo. The blood samples were collected from blood donorscertifed fit on account of negativity for HIV, HBsAg and HCV; at Federal Medical Centre Owo. Sera of participants were analysed using PRNP ELISA kit. Structured questionnaire was used to obtain demographic characteristic and other relevant information for the study. Out of the ninety blood donors screened for variant Creutzfeldt –Jakob disease prion none was found to be seropositive giving overall prevalence rate of 0%. Including screening for variant Creutzfeldt –Jakob disease in the blood safety policy of Nigeria is not advised, as of now. Surveillance should be sustained to review the policy, as the world is a global village.