Abstract: This research determined teachers’ awareness on HIV and AIDS and its relationship to their attitude towards working with a colleague living with the said virus and disease. Utilizing descriptive-correlational research design through the survey method, the study was participated by 150 faculty members from selected basic and higher education institutions in the province of Cavite, Philippines. Results reveal that higher HIV and AIDS awareness among the participants results to a more positive attitude towards working with a colleague with HIV and AIDS. The major scope of this study is the teachers’ level of awareness on HIV and AIDS and their attitude towards working with a colleague with HIV and AIDS. Their awareness on other sexually transmitted infections (STIs) were not included in the study. It solely focused on determining how profound the participants’ knowledge on HIV and AIDS is so that an appropriate health promotion program would be developed for them. The results of this study could serve as a basis in planning for a health promotion program essential in instilling among the the teachers the importance of HIV and AIDS awareness in reducing stigma and discrimination in the workplace.