Abstract: The present study aimed to determine the sensitivity pattern of the isolates against the Tamarindus indica fruit methanolic extracts as well as to detect the mode of action of the extract. Phytoconstituents were obtained from the crude extracts through the process of qualitative mode of screening and antibacterial activity was evaluated by agar well diffusion method against the gram negative bacteria. The bioactive ingredients found were mainly; the alkaloid, flavonoid, tannin, saponins, phenol, and phytosterols were found in the extracts of methanolic leaves which showed sound activities against the tested organisms; E. coli and Shigella. The package used for the data analysis was (SAS) version 8.0.