Background: While most people follow social distancing and are avoiding contact with anyone displaying flu-like symptoms, still asymptomatic patients are having positive SAR COV 2 test can transfer disease to others, as well as the outcome of their disease sill not well understood. Objective: To assess the clinical course of asymptomatic COVID-19 patients who had a positive PCR test in multicenter. Methods: This was a prospective cohort multicenter study conducted in the study was conducted in Khartoum State COVID-19 Centres. Namely Alia Hospital, Fedail, and Ministry of Health Laboratories during the period from December 2020 to April 2021. Data was collected using a questionnaire from 98 patients who fulfilled the inclusion criteria. The questionnaire was filled with patients after taking informed consent. Results: The mean age was 49±7.3 years. Males were 58(59.2%) and females were 40(40.8%). The male to female ratio was 1.5:1. The frequency of symptomatic patients' COVID-19 positive test results experience symptoms was 80(81.6%), while the frequency of asymptomatic patients was 18(18.4%). The time and period of appearance of manifestations among COVID 19 patients, the study indicated that among symptomatic patients after PCR test, the moment of appearance of symptoms was 1 – 7 days in 76(95%) and 8 – 14 days after test in 4(5%). After 28 days follow up the outcome of the patients was recovery 76(76.6%), remain Asymptomatic 18(18.4%), and remain symptomatic 4(4.0%). Conclusion: The study demonstrated that the appearance of symptoms before and after the PCR test was significantly affected by chronic diseases as well as chronic medications in use (P. value <0.05).